Peer Reviewed Publications


I am happy to provide .pdf reprints by e-mail.

  1. Stefan Reinelt, Brigitte Koch, Michael Hothorn, Wolfgang Hengstenberg, Stefan Welti and Klaus Scheffzek
    Structure of the Enterococcus faecalis EIIA(gnt) PTS component
    BBRC, 388(4): 626-629, 2009 [PubMed]

  2. Gyula Timinszky*, Susanne Till*, Paul O. Hassa*, Michael Hothorn*, Georg Kustatscher, Bianca Nijmeijer, Julien Colombelli, Matthias Altmeyer, Ernst H. K. Stelzer, Klaus Scheffzek, Michael O. Hottiger and Andreas G. Ladurner
    A macrodomain-containing histone rearranges chromatin upon sensing PARP1 activation
    Nat Struct Mol Biol, 16(9): 923-929, 2009 [PubMed]
    * these authors contributed equally to this work

  3. Michael Hothorn, Heinz Neumann, Esther D. Lenherr, Mark Wehner, Vladimir Rybin, Paul O. Hassa, Andreas Uttenweiler, Monique Reinhardt, Andrea Schmidt, Jeanette Seiler, Andreas G. Ladurner, Christian Herrmann, Klaus Scheffzek and Andreas Mayer
    Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase
    Science, 324(5926): 513-516, 2009 [PubMed] [Author Manuscript] [Supporting Online Material]

  4. Lorenzo Corsini, Michael Hothorn, Gunter Stier, Vladimir Rybin, Klaus Scheffzek, Toby J. Gibson, and Michael Sattler
    Dimerization and protein binding specificity of the U2AF homology motif (UHM) of the splicing factor Puf60
    J Biol Chem, 284(1): 630-639, 2009 [PubMed], [free full-text]

  5. Lorenzo Corsini, Michael Hothorn, Klaus Scheffzek, Michael Sattler and Gunter Stier
    Thioredoxin as a fusion-tag for carrier-driven crystallization
    Protein Science, 17(12): 2070-2079, 2008 [PubMed], [free full-text]

  6. Tobias Stuwe*, Michael Hothorn*, Erwane Lejeune, Vladimir Rybin, Miriam Bortfeld, Klaus Scheffzek and Andreas G. Ladurner
    The FACT Spt16 "peptidase" domain is a histone H3 H4 binding module
    PNAS, 105(26): 8884-8889, 2008 [Pubmed], [free full-text]
    * these authors contributed equally to this work

  7. Roland Gromes, Michael Hothorn, Esther D. Lenherr, Vladimir Rybin, Klaus Scheffzek and Thomas Rausch
    Redox-switch of gamma-glutamylcysteine ligase via reversible monomer-dimer transition is a mechanism unique to plants
    Plant Journal, 54(6):1063-1075, 2008 [Pubmed]

  8. Vladimir Pena, Michael Hothorn, Alexander Eberth, Nikolai Kaschau, Annabel Parret, Lothar Gremer, Fabien Bonneau, Mohammad Rheza Ahmadian and Klaus Scheffzek
    The C2 domain of SynGAP is essential for stimulation of the Rap GTPase reaction
    EMBO Reports, 9(4):350-355, 2008 [PubMed], [free full-text]

  9. Susanne Till, Erwan Lejeune, Rolf Thermann, Miriam Bortfeld, Michael Hothorn, Daniel Enderle, Constanze Heinrich, Matthias W Hentze and Andreas G. Ladurner
    A conserved motif in Argonaute-interacting proteins mediates functional interactions through the Argonaute PIWI domain
    Nat Struct Mol Biol, 14(10): 897-903, 2007 [PubMed]

  10. Lorenzo Corsini, Sophie Bonnal, Jerome Basquin, Michael Hothorn, Klaus Scheffzek, Juan Valcarcel and Michael Sattler
    U2AF-homology motif interactions are required for alternative splicing regulation by SPF45
    Nat Struct Mol Biol, 14(7): 620-29, 2007 [PubMed]

  11. Hannes Simader, Michael Hothorn and Dietrich Suck
    Structures of the interacting domains from yeast glutamyl-tRNA synthetase and tRNA-aminoacylation and nuclear-export cofactor Arc1p reveal a novel function for an old fold
    Acta Crystallogr D Biol Crystallogr, 62: 1510-19, 2006 [PubMed]

  12. Hannes Simader, Michael Hothorn, Christine Kohler, Jerome Basquin, George Simos and Dietrich Suck
    Structural basis of yeast aminoacyl-tRNA synthetase complex formation revealed by crystal structures of two binary sub-complexes
    Nucleic Acids Res, 34(14): 3968-79, 2006 [PubMed], [free full-text]

  13. Michael Hothorn, Andreas Wachter, Roland Gromes, Tobias Stuwe, Thomas Rausch and Klaus Scheffzek
    Structural basis for the redox-control of plant glutamate cysteine ligase
    J Biol Chem, 281(37): 27557-65, 2006 [PubMed], [free full-text]

  14. Michael Hothorn and Klaus Scheffzek
    Multiple crystal forms of the cell-wall invertase inhibitor from tobacco support high conformational rigidity over a broad pH range
    Acta Crystallogr D Biol Crystallogr, 62(Pt 6): 665-70, 2006 [PubMed]

  15. Georg Kustatscher*, Michael Hothorn*, Celine Pugieux, Klaus Scheffzek and Andreas G. Ladurner
    Splicing regulates NAD metabolite binding to histone macroH2A
    Nat Struct Mol Biol, 12(7): 624-25, 2005 [PubMed]
    * these authors contributed equally to this work

  16. Michael Hothorn, Sebastian Wolf, Patrick Aloy, Steffen Greiner and Klaus Scheffzek
    Structural insights into the target specificity of plant invertase and pectin methylesterase inhibitory proteins
    Plant Cell, 16(12): 3437-47, 2004 [PubMed], [free full-text]

  17. Michael Hothorn, Igor D'Angelo, Jose Antonio Marquez, Steffen Greiner and Klaus Scheffzek
    The invertase inhibitor Nt-CIF from tobacco: a highly thermostable four-helix bundle with an unusual N-terminal extension
    J Mol Biol, 335(4): 987-95, 2004 [PubMed]

  18. Michael Hothorn, Fabien Bonneau, Gunter Stier, Steffen Greiner and Klaus Scheffzek
    Bacterial expression, purification and preliminary X-ray crystallographic characterization of the invertase inhibitor Nt-CIF from tobacco
    Acta Crystallogr D Biol Crystallogr, 59(Pt 12): 2279-82, 2003 [PubMed]

Thesis work

Ph.D. thesis

"Crystallographic studies on protein regulatory mechanisms"
European Molecular Biology Laboratory and University of Heidelberg, Germany, 2006
summa cum laude (with destinction)

Diploma thesis

"Expression, characterisation and crystallisation of a tobacco apoplasmic invertase inhibitor"
Ruperto-Carola University of Heidelberg, Germany, 2002
(with destinction)